Yes, if they're are oriented properly. Once in a while you'll find that a vehicle will drive sideways down the road. Just edit the region file, referenced in your routes config.txt file to add the new car kuids.
Thats what happened to me when I first got TRS2009.... I entered a route to driver and there were trees, houses, bushes, and all kinds of stuff going down the road! I went back to TRS2006.