Are you trying to do a prototypical route (ie, one that exists in real life) and be fairly accurate with it? You may be able to find a track chart that shows signals, turnouts, and other information about the route. Based on your signature I'd assume its somewhere in the Northwest US, so presumably BNSF or UP... I'm not sure how much info is on the site for either of those roads (or sometimes its predecessor companies), but you can try
http://www.multimodalways.org/archives/rrs/rrs.html and see if its under any of the companies involved (You're better off looking under the individual "Carrier" section rather than the first Maps link on the page...) Like I said not sure how much detail they have for BNSF/UP/ATSF/etc... I do know that there is a ton of info for Conrail, CSX, and NS in the northeast (Conrail "Track Charts" are immensely helpful for a lot of North East lines once you determine where the line was "divided" within Conrail's system, if anyone else is looking for assistance with prototyping.)
That being said, it is going to vary from railroad to railroad, but I can tell you that the old New York Central mainline through New York (Conrail called it the "Chicago Line", CSX has a variety of subdivisions within the "Albany" Division on it now) are roughly
two miles apart, varying slightly depending on where the control points (ie, "interlockings" despite not being true interlockings anymore) are located. (ie, if there are two control points within two miles, there are no intermediates or "Autos" between them, if control points are, say, 3 miles apart, there may be an auto in between).
Depending on the era you're aiming for they may be closer together even (when NYC originally put up the signals through here they were only about a mile apart due to shorter/slower running trains)